Show simple item record

2019-12-19Zeitschriftenartikel DOI: 10.3390/app10010033
Photodynamic Opening of the Blood−Brain Barrier Using Different Photosensitizers in Mice
dc.contributor.authorSemyachkina-Glushkovskaya, Oxana
dc.contributor.authorBorisova, Ekaterina
dc.contributor.authorMantareva, Vanya
dc.contributor.authorAngelov, Ivan
dc.contributor.authorEneva, Ivelina
dc.contributor.authorTerskov, Andrey
dc.contributor.authorMamedova, Aysel
dc.contributor.authorShirokov, Alexander
dc.contributor.authorKhorovodov, Alexander
dc.contributor.authorKlimova, Maria
dc.contributor.authorAgranovich, Ilana
dc.contributor.authorBlokhina, Inna
dc.contributor.authorLezhnev, Nikita
dc.contributor.authorKurths, Jurgen
dc.date.accessioned2020-09-11T10:04:50Z
dc.date.available2020-09-11T10:04:50Z
dc.date.issued2019-12-19none
dc.date.updated2020-01-07T19:21:06Z
dc.identifier.urihttp://edoc.hu-berlin.de/18452/22611
dc.description.abstractIn a series of previous studies, we demonstrated that the photodynamic therapy (PDT), as a widely used tool for treatment of glioblastoma multiforme (GBM), also site-specifically opens the blood–brain barrier (BBB) in PDT-dose and age-related manner via reversible disorganization of the tight junction machinery. To develop the effective protocol of PDT-opening of the BBB, here we answer the question of what kind of photosensitizer (PS) is the most effective for the BBB opening. We studied the PDT-opening of the BBB in healthy mice using commercial photosensitizers (PSs) such as 5-aminolevulenic acid (5-ALA), aluminum phthalocyanine disulfonate (AlPcS), zinc phthalocyanine (ZnPc) and new synthetized PSs such as galactose functionalized ZnPc (GalZnPc). The spectrofluorimetric assay of Evans Blue albumin complex (EBAC) leakage and 3-D confocal imaging of FITC-dextran 70 kDa (FITCD) extravasation clearly shows a revisable and dose depended PDT-opening of the BBB to EBAC and FITCD associated with a decrease in presence of tight junction (TJ) in the vascular endothelium. The PDT effects on the BBB permeability, TJ expression and the fluorescent signal from the brain tissues are more pronounced in PDT-GalZnPc vs. PDT-5-ALA/AlPcS/ZnPc. These pre-clinical data are the first important informative platform for an optimization of the PDT protocol in the light of new knowledge about PDT-opening of the BBB for drug brain delivery and for the therapy of brain diseases.eng
dc.description.sponsorshipRussian Science Foundation
dc.description.sponsorshipMinistry of Education and Science of the Russian Federation
dc.description.sponsorshipBulgarian National Science Fund
dc.language.isoengnone
dc.publisherHumboldt-Universität zu Berlin
dc.rights(CC BY 4.0) Attribution 4.0 Internationalger
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectblood–brain barriereng
dc.subjectphotodynamic effectseng
dc.subjectphotosensitizerseng
dc.subjectdrug brain deliveryeng
dc.subject.ddc600 Technik und Technologienone
dc.titlePhotodynamic Opening of the Blood−Brain Barrier Using Different Photosensitizers in Micenone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-110-18452/22611-6
dc.identifier.doi10.3390/app10010033none
dc.identifier.doihttp://dx.doi.org/10.18452/21898
dc.type.versionpublishedVersionnone
local.edoc.container-titleApplied Sciencesnone
local.edoc.pages12none
local.edoc.type-nameZeitschriftenartikel
local.edoc.institutionMathematisch-Naturwissenschaftliche Fakultätnone
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-publisher-nameMDPInone
local.edoc.container-publisher-placeBaselnone
local.edoc.container-volume10none
local.edoc.container-issue1none
dc.description.versionPeer Reviewednone
local.edoc.container-articlenumber33none
dc.identifier.eissn2076-3417
local.edoc.affiliationSemyachkina-Glushkovskaya, Oxana; Saratov National Research State University, 83, Astrakhanskaya str., 410012 Saratov, Russia, glushkovskaya@mail.runone
local.edoc.affiliationBorisova, Ekaterina; Saratov National Research State University, 83, Astrakhanskaya str., 410012 Saratov, Russia, ekaterina.borisova@gmail.com Institute of Electronics, Bulgarian Academy of Sciences, 72, Tsarigradsko Chaussee Blvd., 1784 Sofia, Bulgaria, ekaterina.borisova@gmail.comnone
local.edoc.affiliationMantareva, Vanya; Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev, str., Bld. 9, 1113 Sofia, Bulgaria, mantareva@yahoo.comnone
local.edoc.affiliationAngelov, Ivan; Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev, str., Bld. 9, 1113 Sofia, Bulgaria, ipangelov@gmail.comnone
local.edoc.affiliationEneva, Ivelina; Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev, str., Bld. 9, 1113 Sofia, Bulgaria, ivelina.eneva6@gmail.comnone
local.edoc.affiliationTerskov, Andrey; Saratov National Research State University, 83, Astrakhanskaya str., 410012 Saratov, Russia, terskow.andrey@gmail.comnone
local.edoc.affiliationMamedova, Aysel; Saratov National Research State University, 83, Astrakhanskaya str., 410012 Saratov, Russia, mamedovaysel95@gmail.comnone
local.edoc.affiliationShirokov, Alexander; Saratov National Research State University, 83, Astrakhanskaya str., 410012 Saratov, Russia, shirokov_a@ibppm.ru Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Prospekt Entuziastov 13, 410049 Saratov, Russia, shirokov_a@ibppm.ru Saratov State Medical University, Bolshaya Kazachia st., 112, 410012 Saratov, Russia, shirokov_a@ibppm.runone
local.edoc.affiliationKhorovodov, Alexander; Saratov National Research State University, 83, Astrakhanskaya str., 410012 Saratov, Russia, khorovodov2012@yandex.runone
local.edoc.affiliationKlimova, Maria; Saratov National Research State University, 83, Astrakhanskaya str., 410012 Saratov, Russia, mari-1997@mail.runone
local.edoc.affiliationAgranovich, Ilana; Saratov National Research State University, 83, Astrakhanskaya str., 410012 Saratov, Russia, ilana.agranovich@yandex.runone
local.edoc.affiliationBlokhina, Inna; Saratov National Research State University, 83, Astrakhanskaya str., 410012 Saratov, Russia, inna-474@yandex.runone
local.edoc.affiliationLezhnev, Nikita; Saratov National Research State University, 83, Astrakhanskaya str., 410012 Saratov, Russia, nik.lezhnev.2018@mail.runone
local.edoc.affiliationKurths, Jurgen; Saratov National Research State University, 83, Astrakhanskaya str., 410012 Saratov, Russia, kurths@pik-potsdam.de Physics Department, Humboldt University, Newtonstrasse 15, 12489 Berlin, Germany, kurths@pik-potsdam.de Potsdam Institute for Climate Impact Research, Telegrafenberg A31, 14473 Potsdam, Germany, kurths@pik-potsdam.denone

Show simple item record