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2020-03-11Zeitschriftenartikel DOI: 10.3390/genes11030296
Standards for Methods Utilizing Environmental DNA for Detection of Fish Species
dc.contributor.authorShu, Lu
dc.contributor.authorLudwig, Arne
dc.contributor.authorPeng, Zuogang
dc.date.accessioned2020-10-13T10:13:32Z
dc.date.available2020-10-13T10:13:32Z
dc.date.issued2020-03-11none
dc.date.updated2020-04-28T03:33:49Z
dc.identifier.urihttp://edoc.hu-berlin.de/18452/22714
dc.description.abstractEnvironmental DNA (eDNA) techniques are gaining attention as cost-effective, non-invasive strategies for acquiring information on fish and other aquatic organisms from water samples. Currently, eDNA approaches are used to detect specific fish species and determine fish community diversity. Various protocols used with eDNA methods for aquatic organism detection have been reported in different eDNA studies, but there are no general recommendations for fish detection. Herein, we reviewed 168 papers to supplement and highlight the key criteria for each step of eDNA technology in fish detection and provide general suggestions for eliminating detection errors. Although there is no unified recommendation for the application of diverse eDNA in detecting fish species, in most cases, 1 or 2 L surface water collection and eDNA capture on 0.7-μm glass fiber filters followed by extraction with a DNeasy Blood and Tissue Kit or PowerWater DNA Isolation Kit are useful for obtaining high-quality eDNA. Subsequently, species-specific quantitative polymerase chain reaction (qPCR) assays based on mitochondrial cytochrome b gene markers or eDNA metabarcoding based on both 12S and 16S rRNA markers via high-throughput sequencing can effectively detect target DNA or estimate species richness. Furthermore, detection errors can be minimized by mitigating contamination, negative control, PCR replication, and using multiple genetic markers. Our aim is to provide a useful strategy for fish eDNA technology that can be applied by researchers, advisors, and managers.eng
dc.description.sponsorshipNational Natural Science Foundation of China
dc.language.isoengnone
dc.publisherHumboldt-Universität zu Berlin
dc.rights(CC BY 4.0) Attribution 4.0 Internationalger
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectenvironmental DNAeng
dc.subjectwater samplingeng
dc.subjecteDNA captureeng
dc.subjecteDNA extractioneng
dc.subjecteDNA detectioneng
dc.subjectgenetic markereng
dc.subjectdetection erroreng
dc.subject.ddc570 Biowissenschaften; Biologienone
dc.titleStandards for Methods Utilizing Environmental DNA for Detection of Fish Speciesnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-110-18452/22714-9
dc.identifier.doi10.3390/genes11030296none
dc.identifier.doihttp://dx.doi.org/10.18452/22037
dc.type.versionpublishedVersionnone
local.edoc.container-titleGenesnone
local.edoc.pages14none
local.edoc.type-nameZeitschriftenartikel
local.edoc.institutionLebenswissenschaftliche Fakultätnone
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-publisher-nameMDPInone
local.edoc.container-publisher-placeBaselnone
local.edoc.container-volume11none
local.edoc.container-issue3none
dc.description.versionPeer Reviewednone
local.edoc.container-articlenumber296none
dc.identifier.eissn2073-4425
local.edoc.affiliationShu, Lu; Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Southwest University School of Life Sciences, Chongqing 400715, China, shulu331@email.swu.edu.cnnone
local.edoc.affiliationLudwig, Arne; Department of Evolutionary Genetics, Leibniz-Institute for Zoo and Wildlife Research, 10315 Berlin, Germany, ludwig@izw-berlin.de Albrecht Daniel Thaer-Institute, Faculty of Life Sciences, Humboldt University Berlin, 10115 Berlin, Germany, ludwig@izw-berlin.denone
local.edoc.affiliationPeng, Zuogang; Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Southwest University School of Life Sciences, Chongqing 400715, China, pzg@swu.edu.cnnone

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