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2020-01-31Zeitschriftenartikel DOI: 10.3389/fpls.2020.00002
Characterization of a Plastoglobule-Localized SOUL4 Heme-Binding Protein in Arabidopsis thaliana
dc.contributor.authorShanmugabalaji, Venkatasalam
dc.contributor.authorGrimm, Bernhard
dc.contributor.authorKessler, Felix
dc.date.accessioned2021-03-26T11:15:10Z
dc.date.available2021-03-26T11:15:10Z
dc.date.issued2020-01-31none
dc.date.updated2020-01-31T16:09:04Z
dc.identifier.urihttp://edoc.hu-berlin.de/18452/23227
dc.description.abstractHeme plays an active role in primary plant metabolic pathways as well as in stress signaling. In this study, we characterized the predicted heme-binding protein SOUL4. Proteomics evidence suggests that SOUL4 is a component of Arabidopsis plastoglobules (PGs, chloroplast lipid droplets). SOUL4 contains heme-binding motifs and the recombinant protein is shown here to bind heme in vitro. Fluorescence-tagged SOUL4 colocalized with the specific PG marker Fibrillin1A (FBN1A) in transiently transformed Nicotiana benthamiana leaves. In addition, SOUL4 cofractionated with another PG marker Fibrillin2 (FBN2) in sucrose gradient ultracentrifugation experiments. In vitro kinase experiments revealed that SOUL4 is phosphorylated by a yet unknown chloroplast protein kinase. Our data demonstrate that SOUL4 is a bona fide PG protein and may function in heme-buffering in the chloroplast.eng
dc.language.isoengnone
dc.publisherHumboldt-Universität zu Berlin
dc.rights(CC BY 4.0) Attribution 4.0 Internationalger
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectplastoglobuleeng
dc.subjectSOUL4eng
dc.subjectheme-binding proteineng
dc.subjectchloroplasteng
dc.subjectkinaseeng
dc.subject.ddc580 Pflanzen (Botanik)none
dc.subject.ddc570 Biologienone
dc.titleCharacterization of a Plastoglobule-Localized SOUL4 Heme-Binding Protein in Arabidopsis thaliananone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-110-18452/23227-0
dc.identifier.doi10.3389/fpls.2020.00002none
dc.identifier.doihttp://dx.doi.org/10.18452/22611
dc.type.versionpublishedVersionnone
local.edoc.container-titleFrontiers in Plant Sciencenone
local.edoc.pages9none
local.edoc.type-nameZeitschriftenartikel
local.edoc.institutionLebenswissenschaftliche Fakultätnone
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-publisher-nameFrontiers Media S.A.none
local.edoc.container-publisher-placeLausannenone
local.edoc.container-volume11none
local.edoc.container-issue11none
dc.description.versionPeer Reviewednone
local.edoc.container-articlenumber2none
dc.identifier.eissn1664-462X
local.edoc.affiliationShanmugabalaji, Venkatasalam; 1Laboratory of Plant Physiology, Institute of Biology, University of Neuchâtel, Neuchâtel, Switzerlandnone
local.edoc.affiliationGrimm, Bernhard; 2Institute of Biology/Plant Physiology, Humboldt University Berlin, Berlin, Germanynone
local.edoc.affiliationKessler, Felix; 1Laboratory of Plant Physiology, Institute of Biology, University of Neuchâtel, Neuchâtel, Switzerlandnone

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