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2020-08-14Zeitschriftenartikel DOI: 10.18452/22897
Specialized and shared functions of diguanylate cyclases and phosphodiesterases in Streptomyces development
dc.contributor.authorHaist, Julian
dc.contributor.authorNeumann, Sara Alina
dc.contributor.authorAl-Bassam, Mahmoud
dc.contributor.authorLindenberg, Sandra
dc.contributor.authorElliot, Marie A.
dc.contributor.authorTschowri, Natalia
dc.date.accessioned2021-05-21T07:45:09Z
dc.date.available2021-05-21T07:45:09Z
dc.date.issued2020-08-14none
dc.date.updated2021-03-01T13:01:59Z
dc.identifier.issn0950-382X
dc.identifier.urihttp://edoc.hu-berlin.de/18452/23559
dc.description.abstractThe second messenger bis‐3,5‐cyclic di‐guanosine monophosphate (c‐di‐GMP) determines when Streptomyces initiate sporulation. c‐di‐GMP signals are integrated into the genetic differentiation network by the regulator BldD and the sigma factor σWhiG. However, functions of the development‐specific diguanylate cyclases (DGCs) CdgB and CdgC, and the c‐di‐GMP phosphodiesterases (PDEs) RmdA and RmdB, are poorly understood. Here, we provide biochemical evidence that the GGDEF‐EAL domain protein RmdB from S. venezuelae is a monofunctional PDE that hydrolyzes c‐di‐GMP to 5′pGpG. Despite having an equivalent GGDEF‐EAL domain arrangement, RmdA cleaves c‐di‐GMP to GMP and exhibits residual DGC activity. We show that an intact EAL motif is crucial for the in vivo function of both enzymes since strains expressing protein variants with an AAA motif instead of EAL are delayed in development, similar to null mutants. Transcriptome analysis of ∆cdgB, ∆cdgC, ∆rmdA, and ∆rmdB strains revealed that the c‐di‐GMP specified by these enzymes has a global regulatory role, with about 20% of all S. venezuelae genes being differentially expressed in the cdgC mutant. Our data suggest that the major c‐di‐GMP‐controlled targets determining the timing and mode of sporulation are genes involved in cell division and the production of the hydrophobic sheath that covers Streptomyces aerial hyphae and spores.eng
dc.description.sponsorshipNatural Sciences and Engineering Council of Canada
dc.description.sponsorshipDeutsche Forschungsgemeinschaft
dc.language.isoengnone
dc.publisherHumboldt-Universität zu Berlin
dc.rights(CC BY 4.0) Attribution 4.0 Internationalger
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectc‐di‐GMPeng
dc.subjectdiguanylate cyclaseeng
dc.subjectEALeng
dc.subjectGGDEFeng
dc.subjectphosphodiesteraseeng
dc.subjectStreptomyceseng
dc.subject.ddc570 Biologienone
dc.titleSpecialized and shared functions of diguanylate cyclases and phosphodiesterases in Streptomyces developmentnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-110-18452/23559-8
dc.identifier.doihttp://dx.doi.org/10.18452/22897
dc.type.versionpublishedVersionnone
local.edoc.pages15none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
dc.description.versionPeer Reviewednone
dc.identifier.eissn1365-2958
dcterms.bibliographicCitation.doi10.1111/mmi.14581none
dcterms.bibliographicCitation.journaltitleMolecular microbiologynone
dcterms.bibliographicCitation.volume114none
dcterms.bibliographicCitation.issue5none
dcterms.bibliographicCitation.originalpublishernameWiley-Blackwellnone
dcterms.bibliographicCitation.originalpublisherplaceOxford [u.a.]none
dcterms.bibliographicCitation.pagestart808none
dcterms.bibliographicCitation.pageend822none
bua.import.affiliationHaist, Julian: Department of Biology/Microbiology, Humboldt-Universität zu Berlin, Berlin, Germanynone
bua.import.affiliationNeumann, Sara Alina: Department of Biology/Microbiology, Humboldt-Universität zu Berlin, Berlin, Germanynone
bua.import.affiliationAl-Bassam, Mahmoud M.: Department of Pediatrics, University of California, San Diego, CA, USAnone
bua.import.affiliationLindenberg, Sandra: Department of Biology/Microbiology, Humboldt-Universität zu Berlin, Berlin, Germanynone
bua.import.affiliationElliot, Marie A.: Department of Biology, McMaster University, Hamilton, ON, Canadanone
bua.import.affiliationTschowri, Natalia: Department of Biology/Microbiology, Humboldt-Universität zu Berlin, Berlin, Germanynone
bua.departmentLebenswissenschaftliche Fakultätnone

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