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2021-07-11Zeitschriftenartikel DOI: 10.18452/23363
Development of a Lateral Flow Immunoassay (LFIA) to Screen for the Release of the Endocrine Disruptor Bisphenol A from Polymer Materials and Products
dc.contributor.authorRaysyan, Anna
dc.contributor.authorSchneider, Rudolf J.
dc.date.accessioned2021-09-20T11:27:21Z
dc.date.available2021-09-20T11:27:21Z
dc.date.issued2021-07-11none
dc.date.updated2021-08-01T13:57:23Z
dc.identifier.urihttp://edoc.hu-berlin.de/18452/24013
dc.description.abstractOne of the most important chemicals used in the production of polymer plastics and coatings is bisphenol A. However, despite the large number of studies on the toxicity and hormonal activity of BPA, there are still open questions and thus considerable media attention regarding BPA toxicity. Hence, it is necessary to develop a sensitive, simple, cost-efficient, specific, portable, and rapid method for monitoring bisphenol A and for high sample throughput and on-site screening analysis. Lateral flow immunoassays have potential as rapid tests for on-site screening. To meet sensitivity criteria, they must be carefully optimized. A latex microparticle-based LFIA for detection of BPA was developed. The sensitivity of the assay was improved by non-contact printing of spot grids as the control and test lines with careful parameter optimization. Results of the test could be visually evaluated within 10 min with a visual cut-off of 10 µg/L (vLOD). Alternatively, photographs were taken, and image analysis performed to set up a calibration, which allowed for a calculated limit of detection (cLOD) of 0.14 µg/L. The method was validated for thermal paper samples against ELISA and LC–MS/MS as reference methods, showing good agreement with both methods.eng
dc.description.sponsorshipBundesanstalt für Materialforschung und -Prüfung
dc.language.isoengnone
dc.publisherHumboldt-Universität zu Berlin
dc.rights(CC BY 4.0) Attribution 4.0 Internationalger
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectLFIAeng
dc.subjectimmunoassayeng
dc.subjectbisphenol Aeng
dc.subjectendocrine disruptoreng
dc.subject.ddc570 Biologienone
dc.titleDevelopment of a Lateral Flow Immunoassay (LFIA) to Screen for the Release of the Endocrine Disruptor Bisphenol A from Polymer Materials and Productsnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-110-18452/24013-5
dc.identifier.doihttp://dx.doi.org/10.18452/23363
dc.type.versionpublishedVersionnone
local.edoc.pages11none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
dc.description.versionPeer Reviewednone
dc.identifier.eissn2079-6374
dcterms.bibliographicCitation.doi10.3390/bios11070231none
dcterms.bibliographicCitation.journaltitleBiosensors : open access journalnone
dcterms.bibliographicCitation.volume11none
dcterms.bibliographicCitation.issue7none
dcterms.bibliographicCitation.articlenumber231none
dcterms.bibliographicCitation.originalpublishernameMDPInone
dcterms.bibliographicCitation.originalpublisherplaceBaselnone
bua.departmentMathematisch-Naturwissenschaftliche Fakultätnone

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