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2021-09-27Zeitschriftenartikel DOI: 10.3390/biology10100964
Assessment of Albumin ECM Accumulation and Inflammation as Novel In Vivo Diagnostic Targets for Multi-Target MR Imaging
dc.contributor.authorMöckel, Jana
dc.contributor.authorBrangsch, Julia
dc.contributor.authorReimann, Carolin
dc.contributor.authorKaufmann, Jan O.
dc.contributor.authorSack, Ingolf
dc.contributor.authorMangarova, Dilyana
dc.contributor.authorAvan, Kader
dc.contributor.authorTaupitz, Matthias
dc.contributor.authorAdams, Lisa C.
dc.contributor.authorKeller, Sarah
dc.contributor.authorLudwig, Antje
dc.contributor.authorHamm, Bernd
dc.contributor.authorBotnar, Rene
dc.contributor.authorMakowski, Marcus R.
dc.date.accessioned2021-10-14T09:28:06Z
dc.date.available2021-10-14T09:28:06Z
dc.date.issued2021-09-27none
dc.date.updated2021-10-01T16:31:05Z
dc.identifier.urihttp://edoc.hu-berlin.de/18452/24199
dc.description.abstractAtherosclerosis is a progressive inflammatory vascular disease characterized by endothelial dysfunction and plaque burden. Extracellular matrix (ECM)-associated plasma proteins play an important role in disease development. Our magnetic resonance imaging (MRI) study investigates the feasibility of using two different molecular MRI probes for the simultaneous assessment of ECM-associated intraplaque albumin deposits caused by endothelial damage and progressive inflammation in atherosclerosis. Male apolipoprotein E-deficient (ApoE-/-)-mice were fed a high-fat diet (HFD) for 2 or 4 months. Another ApoE-/--group was treated with pravastatin and received a HFD for 4 months. T1- and T2*-weighted MRI was performed before and after albumin-specific MRI probe (gadofosveset) administration and a macrophage-specific contrast agent (ferumoxytol). Thereafter, laser ablation inductively coupled plasma mass spectrometry and histology were performed. With advancing atherosclerosis, albumin-based MRI signal enhancement and ferumoxytol-induced signal loss areas in T2*-weighted MRI increased. Significant correlations between contrast-to-noise-ratio (CNR) post-gadofosveset and albumin stain (R2 = 0.78, p < 0.05), and signal loss areas in T2*-weighted MRI with Perls’ Prussian blue stain (R2 = 0.83, p < 0.05) were observed. No interference of ferumoxytol with gadofosveset enhancement was detectable. Pravastatin led to decreased inflammation and intraplaque albumin. Multi-target MRI combining ferumoxytol and gadofosveset is a promising method to improve diagnosis and treatment monitoring in atherosclerosis.eng
dc.language.isoengnone
dc.publisherHumboldt-Universität zu Berlin
dc.rights(CC BY 4.0) Attribution 4.0 Internationalger
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectserum albumineng
dc.subjectextracellular matrixeng
dc.subjectmacrophageseng
dc.subjectatherosclerosiseng
dc.subjectmagnetic resonance imagingeng
dc.subject.ddc570 Biologienone
dc.titleAssessment of Albumin ECM Accumulation and Inflammation as Novel In Vivo Diagnostic Targets for Multi-Target MR Imagingnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-110-18452/24199-7
dc.identifier.doi10.3390/biology10100964none
dc.identifier.doihttp://dx.doi.org/10.18452/23541
dc.type.versionpublishedVersionnone
local.edoc.container-titleBiologynone
local.edoc.pages16none
local.edoc.type-nameZeitschriftenartikel
local.edoc.institutionMathematisch-Naturwissenschaftliche Fakultätnone
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-publisher-nameMDPInone
local.edoc.container-publisher-placeBaselnone
local.edoc.container-volume10none
local.edoc.container-issue10none
dc.description.versionPeer Reviewednone
local.edoc.container-articlenumber964none
dc.identifier.eissn2079-7737
local.edoc.affiliationMöckel, Jana; 1Department of Radiology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Charitéplatz 1, 10117 Berlin, Germany; julia.brangsch@charite.de (J.B.); carolinreimann1990@web.de (C.R.); ingolf.sack@charite.de (I.S.); dilyana.mangarova@charite.de (D.B.M.); avan.kader@charite.de (A.K.); matthias.taupitz@charite.de (M.T.); lisa.adams@charite.de (L.C.A.); sarah.keller@charite.de (S.K.); antje.ludwig@charite.de (A.L.); bernd.hamm@charite.de (B.H.); marcus.makowski@tum.de (M.R.M.)none
local.edoc.affiliationBrangsch, Julia; 1Department of Radiology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Charitéplatz 1, 10117 Berlin, Germany; julia.brangsch@charite.de (J.B.); carolinreimann1990@web.de (C.R.); ingolf.sack@charite.de (I.S.); dilyana.mangarova@charite.de (D.B.M.); avan.kader@charite.de (A.K.); matthias.taupitz@charite.de (M.T.); lisa.adams@charite.de (L.C.A.); sarah.keller@charite.de (S.K.); antje.ludwig@charite.de (A.L.); bernd.hamm@charite.de (B.H.); marcus.makowski@tum.de (M.R.M.)none
local.edoc.affiliationReimann, Carolin; 1Department of Radiology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Charitéplatz 1, 10117 Berlin, Germany; julia.brangsch@charite.de (J.B.); carolinreimann1990@web.de (C.R.); ingolf.sack@charite.de (I.S.); dilyana.mangarova@charite.de (D.B.M.); avan.kader@charite.de (A.K.); matthias.taupitz@charite.de (M.T.); lisa.adams@charite.de (L.C.A.); sarah.keller@charite.de (S.K.); antje.ludwig@charite.de (A.L.); bernd.hamm@charite.de (B.H.); marcus.makowski@tum.de (M.R.M.)none
local.edoc.affiliationKaufmann, Jan O.; 3Division 1.5 Protein Analysis, Federal Institute for Materials Research and Testing, Richard-Willstätter-Str. 11, 12489 Berlin, Germany; jan-ole.kaufmann@charite.denone
local.edoc.affiliationSack, Ingolf; 1Department of Radiology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Charitéplatz 1, 10117 Berlin, Germany; julia.brangsch@charite.de (J.B.); carolinreimann1990@web.de (C.R.); ingolf.sack@charite.de (I.S.); dilyana.mangarova@charite.de (D.B.M.); avan.kader@charite.de (A.K.); matthias.taupitz@charite.de (M.T.); lisa.adams@charite.de (L.C.A.); sarah.keller@charite.de (S.K.); antje.ludwig@charite.de (A.L.); bernd.hamm@charite.de (B.H.); marcus.makowski@tum.de (M.R.M.)none
local.edoc.affiliationMangarova, Dilyana B.; 1Department of Radiology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Charitéplatz 1, 10117 Berlin, Germany; julia.brangsch@charite.de (J.B.); carolinreimann1990@web.de (C.R.); ingolf.sack@charite.de (I.S.); dilyana.mangarova@charite.de (D.B.M.); avan.kader@charite.de (A.K.); matthias.taupitz@charite.de (M.T.); lisa.adams@charite.de (L.C.A.); sarah.keller@charite.de (S.K.); antje.ludwig@charite.de (A.L.); bernd.hamm@charite.de (B.H.); marcus.makowski@tum.de (M.R.M.)none
local.edoc.affiliationKader, Avan; 1Department of Radiology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Charitéplatz 1, 10117 Berlin, Germany; julia.brangsch@charite.de (J.B.); carolinreimann1990@web.de (C.R.); ingolf.sack@charite.de (I.S.); dilyana.mangarova@charite.de (D.B.M.); avan.kader@charite.de (A.K.); matthias.taupitz@charite.de (M.T.); lisa.adams@charite.de (L.C.A.); sarah.keller@charite.de (S.K.); antje.ludwig@charite.de (A.L.); bernd.hamm@charite.de (B.H.); marcus.makowski@tum.de (M.R.M.)none
local.edoc.affiliationTaupitz, Matthias; 1Department of Radiology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Charitéplatz 1, 10117 Berlin, Germany; julia.brangsch@charite.de (J.B.); carolinreimann1990@web.de (C.R.); ingolf.sack@charite.de (I.S.); dilyana.mangarova@charite.de (D.B.M.); avan.kader@charite.de (A.K.); matthias.taupitz@charite.de (M.T.); lisa.adams@charite.de (L.C.A.); sarah.keller@charite.de (S.K.); antje.ludwig@charite.de (A.L.); bernd.hamm@charite.de (B.H.); marcus.makowski@tum.de (M.R.M.)none
local.edoc.affiliationAdams, Lisa C.; 1Department of Radiology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Charitéplatz 1, 10117 Berlin, Germany; julia.brangsch@charite.de (J.B.); carolinreimann1990@web.de (C.R.); ingolf.sack@charite.de (I.S.); dilyana.mangarova@charite.de (D.B.M.); avan.kader@charite.de (A.K.); matthias.taupitz@charite.de (M.T.); lisa.adams@charite.de (L.C.A.); sarah.keller@charite.de (S.K.); antje.ludwig@charite.de (A.L.); bernd.hamm@charite.de (B.H.); marcus.makowski@tum.de (M.R.M.)none
local.edoc.affiliationKeller, Sarah; 1Department of Radiology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Charitéplatz 1, 10117 Berlin, Germany; julia.brangsch@charite.de (J.B.); carolinreimann1990@web.de (C.R.); ingolf.sack@charite.de (I.S.); dilyana.mangarova@charite.de (D.B.M.); avan.kader@charite.de (A.K.); matthias.taupitz@charite.de (M.T.); lisa.adams@charite.de (L.C.A.); sarah.keller@charite.de (S.K.); antje.ludwig@charite.de (A.L.); bernd.hamm@charite.de (B.H.); marcus.makowski@tum.de (M.R.M.)none
local.edoc.affiliationLudwig, Antje; 1Department of Radiology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Charitéplatz 1, 10117 Berlin, Germany; julia.brangsch@charite.de (J.B.); carolinreimann1990@web.de (C.R.); ingolf.sack@charite.de (I.S.); dilyana.mangarova@charite.de (D.B.M.); avan.kader@charite.de (A.K.); matthias.taupitz@charite.de (M.T.); lisa.adams@charite.de (L.C.A.); sarah.keller@charite.de (S.K.); antje.ludwig@charite.de (A.L.); bernd.hamm@charite.de (B.H.); marcus.makowski@tum.de (M.R.M.)none
local.edoc.affiliationHamm, Bernd; 1Department of Radiology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Charitéplatz 1, 10117 Berlin, Germany; julia.brangsch@charite.de (J.B.); carolinreimann1990@web.de (C.R.); ingolf.sack@charite.de (I.S.); dilyana.mangarova@charite.de (D.B.M.); avan.kader@charite.de (A.K.); matthias.taupitz@charite.de (M.T.); lisa.adams@charite.de (L.C.A.); sarah.keller@charite.de (S.K.); antje.ludwig@charite.de (A.L.); bernd.hamm@charite.de (B.H.); marcus.makowski@tum.de (M.R.M.)none
local.edoc.affiliationBotnar, Rene M.; 9Wellcome Trust/EPSRC Centre for Medical Engineering, King’s College London, London WC2R 2LS, UK; rene.botnar@kcl.ac.uknone
local.edoc.affiliationMakowski, Marcus R.; 1Department of Radiology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Charitéplatz 1, 10117 Berlin, Germany; julia.brangsch@charite.de (J.B.); carolinreimann1990@web.de (C.R.); ingolf.sack@charite.de (I.S.); dilyana.mangarova@charite.de (D.B.M.); avan.kader@charite.de (A.K.); matthias.taupitz@charite.de (M.T.); lisa.adams@charite.de (L.C.A.); sarah.keller@charite.de (S.K.); antje.ludwig@charite.de (A.L.); bernd.hamm@charite.de (B.H.); marcus.makowski@tum.de (M.R.M.)none

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