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2021-07-12Zeitschriftenartikel DOI: 10.1002/celc.202100446
Electrochemical Immunomagnetic Ochratoxin A Sensing: Steps Forward in the Application of 3,3’,5,5’‐Tetramethylbenzidine in Amperometric Assays
dc.contributor.authorHöfs, Soraya
dc.contributor.authorHülagü, Deniz
dc.contributor.authorBennet, Francesca
dc.contributor.authorCarl, Peter
dc.contributor.authorFlemig, Sabine
dc.contributor.authorSchmid, Thomas
dc.contributor.authorSchenk, Jörg
dc.contributor.authorHodoroaba, Vasile-Dan
dc.contributor.authorSchneider, Rudolf J.
dc.date.accessioned2021-10-15T12:18:52Z
dc.date.available2021-10-15T12:18:52Z
dc.date.issued2021-07-12none
dc.date.updated2021-09-08T12:10:31Z
dc.identifier.urihttp://edoc.hu-berlin.de/18452/24224
dc.description.abstractElectrochemical methods offer great promise in meeting the demand for user-friendly on-site devices for monitoring important parameters. The food industry often runs own lab procedures, for example, for mycotoxin analysis, but it is a major goal to simplify analysis, linking analytical methods with smart technologies. Enzyme-linked immunosorbent assays, with photometric detection of 3,3’,5,5’-tetramethylbenzidine (TMB), form a good basis for sensitive detection. To provide a straightforward approach for the miniaturization of the detection step, we have studied the pitfalls of the electrochemical TMB detection. By cyclic voltammetry it was found that the TMB electrochemistry is strongly dependent on the pH and the electrode material. A stable electrode response to TMB could be achieved at pH 1 on gold electrodes. We created a smartphone-based, electrochemical, immunomagnetic assay for the detection of ochratoxin A in real samples, providing a solid basis for sensing of further analytes.eng
dc.description.sponsorshipBAM
dc.description.sponsorshipGerman Federal Ministry of Education and Research (BMBF)
dc.language.isoengnone
dc.publisherHumboldt-Universität zu Berlin
dc.rights(CC BY 4.0) Attribution 4.0 Internationalger
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectamperometryeng
dc.subjectcyclic voltammetryeng
dc.subjectimmunoassayseng
dc.subjectscreen-printed electrodeseng
dc.subject3,3′,5,5′-tetramethylbenzidineeng
dc.subject.ddc540 Chemie und zugeordnete Wissenschaftennone
dc.titleElectrochemical Immunomagnetic Ochratoxin A Sensing: Steps Forward in the Application of 3,3’,5,5’‐Tetramethylbenzidine in Amperometric Assaysnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-110-18452/24224-8
dc.identifier.doi10.1002/celc.202100446none
dc.identifier.doihttp://dx.doi.org/10.18452/23568
dc.type.versionpublishedVersionnone
local.edoc.container-titleChemElectroChemnone
local.edoc.type-nameZeitschriftenartikel
local.edoc.institutionHumboldt-Universität (insgesamt)none
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-publisher-nameWiley-VCHnone
local.edoc.container-publisher-placeWeinheimnone
local.edoc.container-volume8none
local.edoc.container-issue13none
local.edoc.container-firstpage2597none
local.edoc.container-lastpage2606none
dc.description.versionPeer Reviewednone
dc.identifier.eissn2196-0216
local.edoc.affiliationHöfs, Soraya; 1 Department of Analytical Chemistry Reference Materials Bundesanstalt für Materialforschung und -prüfung (BAM) Richard-Willstätter-Straße 11 12489 Berlin Germanynone
local.edoc.affiliationHülagü, Deniz; 3 Department of Materials Chemistry Bundesanstalt für Materialforschung und -prüfung (BAM) Unter den Eichen 44–46 12203 Berlin Germanynone
local.edoc.affiliationBennet, Francesca; 3 Department of Materials Chemistry Bundesanstalt für Materialforschung und -prüfung (BAM) Unter den Eichen 44–46 12203 Berlin Germanynone
local.edoc.affiliationCarl, Peter; 1 Department of Analytical Chemistry Reference Materials Bundesanstalt für Materialforschung und -prüfung (BAM) Richard-Willstätter-Straße 11 12489 Berlin Germanynone
local.edoc.affiliationFlemig, Sabine; 1 Department of Analytical Chemistry Reference Materials Bundesanstalt für Materialforschung und -prüfung (BAM) Richard-Willstätter-Straße 11 12489 Berlin Germanynone
local.edoc.affiliationSchmid, Thomas; 1 Department of Analytical Chemistry Reference Materials Bundesanstalt für Materialforschung und -prüfung (BAM) Richard-Willstätter-Straße 11 12489 Berlin Germanynone
local.edoc.affiliationSchenk, Jörg A.; 5 Hybrotec GmbH Am Mühlenberg 11 14476 Potsdam Germanynone
local.edoc.affiliationHodoroaba, Vasile‐Dan; 3 Department of Materials Chemistry Bundesanstalt für Materialforschung und -prüfung (BAM) Unter den Eichen 44–46 12203 Berlin Germanynone
local.edoc.affiliationSchneider, Rudolf J.; 1 Department of Analytical Chemistry Reference Materials Bundesanstalt für Materialforschung und -prüfung (BAM) Richard-Willstätter-Straße 11 12489 Berlin Germanynone

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