Electrochemical Immunomagnetic Ochratoxin A Sensing: Steps Forward in the Application of 3,3’,5,5’‐Tetramethylbenzidine in Amperometric Assays
dc.contributor.author | Höfs, Soraya | |
dc.contributor.author | Hülagü, Deniz | |
dc.contributor.author | Bennet, Francesca | |
dc.contributor.author | Carl, Peter | |
dc.contributor.author | Flemig, Sabine | |
dc.contributor.author | Schmid, Thomas | |
dc.contributor.author | Schenk, Jörg | |
dc.contributor.author | Hodoroaba, Vasile-Dan | |
dc.contributor.author | Schneider, Rudolf J. | |
dc.date.accessioned | 2021-10-15T12:18:52Z | |
dc.date.available | 2021-10-15T12:18:52Z | |
dc.date.issued | 2021-07-12 | none |
dc.date.updated | 2021-09-08T12:10:31Z | |
dc.identifier.uri | http://edoc.hu-berlin.de/18452/24224 | |
dc.description.abstract | Electrochemical methods offer great promise in meeting the demand for user-friendly on-site devices for monitoring important parameters. The food industry often runs own lab procedures, for example, for mycotoxin analysis, but it is a major goal to simplify analysis, linking analytical methods with smart technologies. Enzyme-linked immunosorbent assays, with photometric detection of 3,3’,5,5’-tetramethylbenzidine (TMB), form a good basis for sensitive detection. To provide a straightforward approach for the miniaturization of the detection step, we have studied the pitfalls of the electrochemical TMB detection. By cyclic voltammetry it was found that the TMB electrochemistry is strongly dependent on the pH and the electrode material. A stable electrode response to TMB could be achieved at pH 1 on gold electrodes. We created a smartphone-based, electrochemical, immunomagnetic assay for the detection of ochratoxin A in real samples, providing a solid basis for sensing of further analytes. | eng |
dc.description.sponsorship | BAM | |
dc.description.sponsorship | German Federal Ministry of Education and Research (BMBF) | |
dc.language.iso | eng | none |
dc.publisher | Humboldt-Universität zu Berlin | |
dc.rights | (CC BY 4.0) Attribution 4.0 International | ger |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.subject | amperometry | eng |
dc.subject | cyclic voltammetry | eng |
dc.subject | immunoassays | eng |
dc.subject | screen-printed electrodes | eng |
dc.subject | 3,3′,5,5′-tetramethylbenzidine | eng |
dc.subject.ddc | 540 Chemie und zugeordnete Wissenschaften | none |
dc.title | Electrochemical Immunomagnetic Ochratoxin A Sensing: Steps Forward in the Application of 3,3’,5,5’‐Tetramethylbenzidine in Amperometric Assays | none |
dc.type | article | |
dc.identifier.urn | urn:nbn:de:kobv:11-110-18452/24224-8 | |
dc.identifier.doi | http://dx.doi.org/10.18452/23568 | |
dc.type.version | publishedVersion | none |
local.edoc.type-name | Zeitschriftenartikel | |
local.edoc.container-type | periodical | |
local.edoc.container-type-name | Zeitschrift | |
dc.description.version | Peer Reviewed | none |
dc.identifier.eissn | 2196-0216 | |
dcterms.bibliographicCitation.doi | 10.1002/celc.202100446 | none |
dcterms.bibliographicCitation.journaltitle | ChemElectroChem | none |
dcterms.bibliographicCitation.volume | 8 | none |
dcterms.bibliographicCitation.issue | 13 | none |
dcterms.bibliographicCitation.originalpublishername | Wiley-VCH | none |
dcterms.bibliographicCitation.originalpublisherplace | Weinheim | none |
dcterms.bibliographicCitation.pagestart | 2597 | none |
dcterms.bibliographicCitation.pageend | 2606 | none |
bua.import.affiliation | Höfs, Soraya; 1 Department of Analytical Chemistry Reference Materials Bundesanstalt für Materialforschung und -prüfung (BAM) Richard-Willstätter-Straße 11 12489 Berlin Germany | none |
bua.import.affiliation | Hülagü, Deniz; 3 Department of Materials Chemistry Bundesanstalt für Materialforschung und -prüfung (BAM) Unter den Eichen 44–46 12203 Berlin Germany | none |
bua.import.affiliation | Bennet, Francesca; 3 Department of Materials Chemistry Bundesanstalt für Materialforschung und -prüfung (BAM) Unter den Eichen 44–46 12203 Berlin Germany | none |
bua.import.affiliation | Carl, Peter; 1 Department of Analytical Chemistry Reference Materials Bundesanstalt für Materialforschung und -prüfung (BAM) Richard-Willstätter-Straße 11 12489 Berlin Germany | none |
bua.import.affiliation | Flemig, Sabine; 1 Department of Analytical Chemistry Reference Materials Bundesanstalt für Materialforschung und -prüfung (BAM) Richard-Willstätter-Straße 11 12489 Berlin Germany | none |
bua.import.affiliation | Schmid, Thomas; 1 Department of Analytical Chemistry Reference Materials Bundesanstalt für Materialforschung und -prüfung (BAM) Richard-Willstätter-Straße 11 12489 Berlin Germany | none |
bua.import.affiliation | Schenk, Jörg A.; 5 Hybrotec GmbH Am Mühlenberg 11 14476 Potsdam Germany | none |
bua.import.affiliation | Hodoroaba, Vasile‐Dan; 3 Department of Materials Chemistry Bundesanstalt für Materialforschung und -prüfung (BAM) Unter den Eichen 44–46 12203 Berlin Germany | none |
bua.import.affiliation | Schneider, Rudolf J.; 1 Department of Analytical Chemistry Reference Materials Bundesanstalt für Materialforschung und -prüfung (BAM) Richard-Willstätter-Straße 11 12489 Berlin Germany | none |
bua.department | Humboldt-Universität (insgesamt) | none |