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2021-10-01Zeitschriftenartikel DOI: 10.3389/fpls.2021.732608
Mutation of the ALBOSTRIANS Ohnologous Gene HvCMF3 Impairs Chloroplast Development and Thylakoid Architecture in Barley
dc.contributor.authorLi, Mingjiu
dc.contributor.authorHensel, Goetz
dc.contributor.authorMelzer, Michael
dc.contributor.authorJunker, Astrid
dc.contributor.authorTschiersch, Henning
dc.contributor.authorRuwe, Hannes
dc.contributor.authorArend, Daniel
dc.contributor.authorKumlehn, Jochen
dc.contributor.authorBörner, Thomas
dc.contributor.authorStein, Nils
dc.date.accessioned2021-10-19T11:20:52Z
dc.date.available2021-10-19T11:20:52Z
dc.date.issued2021-10-01none
dc.date.updated2021-10-15T10:30:29Z
dc.identifier.urihttp://edoc.hu-berlin.de/18452/24235
dc.description.abstractGene pairs resulting from whole genome duplication (WGD), so-called ohnologous genes, are retained if at least one member of the pair undergoes neo- or sub-functionalization. Phylogenetic analyses of the ohnologous genes ALBOSTRIANS (HvAST/HvCMF7) and ALBOSTRIANS-LIKE (HvASL/HvCMF3) of barley (Hordeum vulgare) revealed them as members of a subfamily of genes coding for CCT motif (CONSTANS, CONSTANS-LIKE and TIMING OF CAB1) proteins characterized by a single CCT domain and a putative N-terminal chloroplast transit peptide. Recently, we showed that HvCMF7 is needed for chloroplast ribosome biogenesis. Here we demonstrate that mutations in HvCMF3 lead to seedlings delayed in development. They exhibit a yellowish/light green – xantha – phenotype and successively develop pale green leaves. Compared to wild type, plastids of mutant seedlings show a decreased PSII efficiency, impaired processing and reduced amounts of ribosomal RNAs; they contain less thylakoids and grana with a higher number of more loosely stacked thylakoid membranes. Site-directed mutagenesis of HvCMF3 identified a previously unknown functional domain, which is highly conserved within this subfamily of CCT domain containing proteins. HvCMF3:GFP fusion constructs were localized to plastids and nucleus. Hvcmf3Hvcmf7 double mutants exhibited a xantha-albino or albino phenotype depending on the strength of molecular lesion of the HvCMF7 allele. The chloroplast ribosome deficiency is discussed as the primary observed defect of the Hvcmf3 mutants. Based on our observations, the genes HvCMF3 and HvCMF7 have similar but not identical functions in chloroplast development of barley supporting our hypothesis of neo-/sub-functionalization between both ohnologous genes.eng
dc.language.isoengnone
dc.publisherHumboldt-Universität zu Berlin
dc.rights(CC BY 4.0) Attribution 4.0 Internationalger
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectalbostrianseng
dc.subjectchloroplast biogenesiseng
dc.subjectchloroplast translationeng
dc.subjectHordeum vulgareeng
dc.subjectphotosynthesiseng
dc.subjectplant phenotypingeng
dc.subjectrRNA processingeng
dc.subjectreverse geneticseng
dc.subject.ddc570 Biologienone
dc.titleMutation of the ALBOSTRIANS Ohnologous Gene HvCMF3 Impairs Chloroplast Development and Thylakoid Architecture in Barleynone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-110-18452/24235-0
dc.identifier.doi10.3389/fpls.2021.732608none
dc.identifier.doihttp://dx.doi.org/10.18452/23582
dc.type.versionpublishedVersionnone
local.edoc.container-titleFrontiers in plant sciencenone
local.edoc.pages23none
local.edoc.type-nameZeitschriftenartikel
local.edoc.institutionLebenswissenschaftliche Fakultätnone
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-publisher-nameFrontiers Medianone
local.edoc.container-publisher-placeLausannenone
local.edoc.container-volume12none
dc.description.versionPeer Reviewednone
local.edoc.container-articlenumber732608none
dc.identifier.eissn1664-462X
local.edoc.affiliationLi, Mingjiu; 1Genomics of Genetic Resources, Department of Genebank, Leibniz Institute of Plant Genetics and Crop Plant Research, Seeland, Germanynone
local.edoc.affiliationHensel, Goetz; 2Plant Reproductive Biology, Department of Physiology and Cell Biology, Leibniz Institute of Plant Genetics and Crop Plant Research, Seeland, Germanynone
local.edoc.affiliationMelzer, Michael; 3Structural Cell Biology, Department of Physiology and Cell Biology, Leibniz Institute of Plant Genetics and Crop Plant Research, Seeland, Germanynone
local.edoc.affiliationJunker, Astrid; 4Acclimation Dynamics and Phenotyping, Department of Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research, Seeland, Germanynone
local.edoc.affiliationTschiersch, Henning; 5Heterosis Research Group, Department of Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research, Seeland, Germanynone
local.edoc.affiliationRuwe, Hannes; 6Molecular Genetics, Institute of Biology, Humboldt University, Berlin, Germanynone
local.edoc.affiliationArend, Daniel; 7Research Group Bioinformatics and Information Technology, Department of Breeding Research, Leibniz Institute of Plant Genetics and Crop Plant Research, Seeland, Germanynone
local.edoc.affiliationKumlehn, Jochen; 2Plant Reproductive Biology, Department of Physiology and Cell Biology, Leibniz Institute of Plant Genetics and Crop Plant Research, Seeland, Germanynone
local.edoc.affiliationBörner, Thomas; 6Molecular Genetics, Institute of Biology, Humboldt University, Berlin, Germanynone
local.edoc.affiliationStein, Nils; 1Genomics of Genetic Resources, Department of Genebank, Leibniz Institute of Plant Genetics and Crop Plant Research, Seeland, Germanynone

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