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2020-08-28Zeitschriftenartikel DOI: 10.18452/24087
Selective flexible packaging pathways of the segmented genome of influenza A virus
Haralampiev, Ivan
Prisner, Simon
Nitzan, Mor cc
Schade, Matthias cc
Jolmes, Fabian cc
Schreiber, Max cc
Loidolt-Krüger, Maria
Jongen, Kalle
Chamiolo, Jasmine
Nilson, Niklaas
Winter, Franziska cc
Friedman, Nir cc
Seitz, Oliver cc
Wolff, Thorsten cc
Herrmann, Andreas cc
Mathematisch-Naturwissenschaftliche Fakultät
The genome of influenza A viruses (IAV) is encoded in eight distinct viral ribonucleoproteins (vRNPs) that consist of negative sense viral RNA (vRNA) covered by the IAV nucleoprotein. Previous studies strongly support a selective packaging model by which vRNP segments are bundling to an octameric complex, which is integrated into budding virions. However, the pathway(s) generating a complete genome bundle is not known. We here use a multiplexed FISH assay to monitor all eight vRNAs in parallel in human lung epithelial cells. Analysis of 3.9 × 105 spots of colocalizing vRNAs provides quantitative insights into segment composition of vRNP complexes and, thus, implications for bundling routes. The complexes rarely contain multiple copies of a specific segment. The data suggest a selective packaging mechanism with limited flexibility by which vRNPs assemble into a complete IAV genome. We surmise that this flexibility forms an essential basis for the development of reassortant viruses with pandemic potential.
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This article was supported by the German Research Foundation (DFG) and the Open Access Publication Fund of Humboldt-Universität zu Berlin.
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(CC BY 4.0) Attribution 4.0 International(CC BY 4.0) Attribution 4.0 International
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DOI
10.18452/24087
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https://doi.org/10.18452/24087
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