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2021-11-22Zeitschriftenartikel DOI: 10.3390/biology10111217
Visualization and Quantification of the Extracellular Matrix in Prostate Cancer Using an Elastin Specific Molecular Probe
dc.contributor.authorAvan, Kader
dc.contributor.authorBrangsch, Julia
dc.contributor.authorReimann, Carolin
dc.contributor.authorKaufmann, Jan O.
dc.contributor.authorMangarova, Dilyana
dc.contributor.authorMoeckel, Jana
dc.contributor.authorAdams, Lisa C.
dc.contributor.authorZhao, Jing
dc.contributor.authorSaatz, Jessica
dc.contributor.authorTraub, Heike
dc.contributor.authorBuchholz, Rebecca
dc.contributor.authorKarst, Uwe
dc.contributor.authorHamm, Bernd
dc.contributor.authorMakowski, Marcus R.
dc.date.accessioned2022-02-10T13:01:17Z
dc.date.available2022-02-10T13:01:17Z
dc.date.issued2021-11-22none
dc.date.updated2021-12-01T15:14:08Z
dc.identifier.urihttp://edoc.hu-berlin.de/18452/24779
dc.description.abstractHuman prostate cancer (PCa) is a type of malignancy and one of the most frequently diagnosed cancers in men. Elastin is an important component of the extracellular matrix and is involved in the structure and organization of prostate tissue. The present study examined prostate cancer in a xenograft mouse model using an elastin-specific molecular probe for magnetic resonance molecular imaging. Two different tumor sizes (500 mm3 and 1000 mm3) were compared and analyzed by MRI in vivo and histologically and analytically ex vivo. The T1-weighted sequence was used in a clinical 3-T scanner to calculate the relative contrast enhancement before and after probe administration. Our results show that the use of an elastin-specific probe enables better discrimination between tumors and surrounding healthy tissue. Furthermore, specific binding of the probe to elastin fibers was confirmed by histological examination and laser ablation–inductively coupled plasma–mass spectrometry (LA-ICP-MS). Smaller tumors showed significantly higher signal intensity (p > 0.001), which correlates with the higher proportion of elastin fibers in the histological evaluation than in larger tumors. A strong correlation was seen between relative enhancement (RE) and Elastica–van Gieson staining (R2 = 0.88). RE was related to inductively coupled plasma–mass spectrometry data for Gd and showed a correlation (R2 = 0.78). Thus, molecular MRI could become a novel quantitative tool for the early evaluation and detection of PCa.eng
dc.language.isoengnone
dc.publisherHumboldt-Universität zu Berlin
dc.rights(CC BY 4.0) Attribution 4.0 Internationalger
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectmagnetic resonance imagingeng
dc.subjectmolecular imagingeng
dc.subjectprostate cancereng
dc.subject.ddc570 Biologienone
dc.titleVisualization and Quantification of the Extracellular Matrix in Prostate Cancer Using an Elastin Specific Molecular Probenone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-110-18452/24779-7
dc.identifier.doi10.3390/biology10111217none
dc.identifier.doihttp://dx.doi.org/10.18452/24127
dc.type.versionpublishedVersionnone
local.edoc.container-titleBiology : open access journalnone
local.edoc.pages14none
local.edoc.type-nameZeitschriftenartikel
local.edoc.institutionMathematisch-Naturwissenschaftliche Fakultätnone
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-publisher-nameMDPInone
local.edoc.container-publisher-placeBaselnone
local.edoc.container-volume10none
local.edoc.container-issue11none
dc.description.versionPeer Reviewednone
local.edoc.container-articlenumber1217none
dc.identifier.eissn2079-7737

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