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2021-07-03Zeitschriftenartikel

Exogenous Applications of Bio-fabricated Silver Nanoparticles to Improve Biochemical, Antioxidant, Fatty Acid and Secondary Metabolite Contents of Sunflower

Batool, Syeda Umber; Javed, Bilal; Sohail; Zehra, Syeda Sadaf; Mashwani, Zia-ur-Rehman; Raja, Naveed Iqbal; Khan, Tariq; ALHaithloul, Haifa Abdulaziz Sakit; Alghanem, Suliman Mohammed; Al-Mushhin, Amina A. M.; Hashem, Mohamed; Alamri, Saad

The present study involved the bio-fabrication of silver nanoparticles (AgNPs) by using the Euphorbia helioscopia L. leaves aqueous extract to improve the production of secondary metabolites in industrially important ...

2017-10-05Zeitschriftenartikel

Absorption and Emission Spectroscopic Investigation of Thermal Dynamics and Photo-Dynamics of the Rhodopsin Domain of the Rhodopsin-Guanylyl Cyclase from the Nematophagous Fungus Catenaria anguillulae

Penzkofer, Alfons; Scheib, Ulrike; Stehfest, Katja; Hegemann, Peter

The rhodopsin-guanylyl cyclase from the nematophagous fungus Catenaria anguillulae belongs to a recently discovered class of enzymerhodopsins and may find application as a tool in optogenetics. Here the rhodopsin domain ...

2019-08-21Zeitschriftenartikel

Absorption and Emission Spectroscopic Investigation of the Thermal Dynamics of the Archaerhodopsin 3 Based Fluorescent Voltage Sensor QuasAr1

Penzkofer, Alfons; Silapetere, Arita; Hegemann, Peter

QuasAr1 is a fluorescent voltage sensor derived from Archaerhodopsin 3 (Arch) of Halorubrum sodomense by directed evolution. Here we report absorption and emission spectroscopic studies of QuasAr1 in Tris buffer at pH 8. ...

2021-06-23Zeitschriftenartikel

Comparative Anti-Inflammatory Effects of Salix Cortex Extracts and Acetylsalicylic Acid in SARS-CoV-2 Peptide and LPS-Activated Human In Vitro Systems

Le, Nguyen Phan Khoi; Herz, Corinna; Gomes, João Victor Dutra; Förster, Nadja; Antoniadou, Kyriaki; Mittermeier-Kleßinger, Verena Karolin; Mewis, Inga; Dawid, Corinna; Ulrichs, Christian; Lamy, Evelyn

The usefulness of anti-inflammatory drugs as an adjunct therapy to improve outcomes in COVID-19 patients is intensely discussed. Willow bark (Salix cortex) has been used for centuries to relieve pain, inflammation, and ...

2019-12-25Zeitschriftenartikel

Photocycle Dynamics of the Archaerhodopsin 3 Based Fluorescent Voltage Sensor QuasAr1

Penzkofer, Alfons; Silapetere, Arita; Hegemann, Peter

The retinal photocycle dynamics of the fluorescent voltage sensor QuasAr1 (Archaerhodopsin 3 P60S-T80S-D95H-D106H-F161V mutant from Halorubrum sodomense) in pH 8 Tris buffer was studied. The samples were photoexcited to ...

2020-09-08Zeitschriftenartikel

Absorption and Emission Spectroscopic Investigation of the Thermal Dynamics of the Archaerhodopsin 3 Based Fluorescent Voltage Sensor Archon2

Penzkofer, Alfons; Silapetere, Arita; Hegemann, Peter

Archon2 is a fluorescent voltage sensor derived from Archaerhodopsin 3 (Arch) of Halorubrum sodomense using robotic multidimensional directed evolution approach. Here we report absorption and emission spectroscopic studies ...

2019-05-11Zeitschriftenartikel

Computational Aminoacyl-tRNA Synthetase Library Design for Photocaged Tyrosine

Baumann, Tobias; Hauf, Matthias; Richter, Florian; Albers, Suki; Möglich, Andreas; Ignatova, Zoya; Budisa, Nediljko

Engineering aminoacyl-tRNA synthetases (aaRSs) provides access to the ribosomal incorporation of noncanonical amino acids via genetic code expansion. Conventional targeted mutagenesis libraries with 5–7 positions randomized ...