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2022-02-03Zeitschriftenartikel DOI: 10.3389/fmicb.2021.757812
Permafrost Active Layer Microbes From Ny Ålesund, Svalbard (79°N) Show Autotrophic and Heterotrophic Metabolisms With Diverse Carbon-Degrading Enzymes
dc.contributor.authorSipes, Katie
dc.contributor.authorPaul, Raegan
dc.contributor.authorFine, Aubrey
dc.contributor.authorLi, Peibo
dc.contributor.authorLiang, Renxing
dc.contributor.authorBoike, Julia
dc.contributor.authorOnstott, Tullis
dc.contributor.authorVishnivetskaya, Tatiana A.
dc.contributor.authorSchaeffer, Sean
dc.contributor.authorLloyd, Karen
dc.date.accessioned2022-03-31T13:28:50Z
dc.date.available2022-03-31T13:28:50Z
dc.date.issued2022-02-03none
dc.date.updated2022-02-20T04:38:06Z
dc.identifier.urihttp://edoc.hu-berlin.de/18452/25086
dc.description.abstractThe active layer of permafrost in Ny Ålesund, Svalbard (79°N) around the Bayelva River in the Leirhaugen glacier moraine is measured as a small net carbon sink at the brink of becoming a carbon source. In many permafrost-dominating ecosystems, microbes in the active layers have been shown to drive organic matter degradation and greenhouse gas production, creating positive feedback on climate change. However, the microbial metabolisms linking the environmental geochemical processes and the populations that perform them have not been fully characterized. In this paper, we present geochemical, enzymatic, and isotopic data paired with 10 Pseudomonas sp. cultures and metagenomic libraries of two active layer soil cores (BPF1 and BPF2) from Ny Ålesund, Svalbard, (79°N). Relative to BPF1, BPF2 had statistically higher C/N ratios (15 ± 1 for BPF1 vs. 29 ± 10 for BPF2; n = 30, p < 10–5), statistically lower organic carbon (2% ± 0.6% for BPF1 vs. 1.6% ± 0.4% for BPF2, p < 0.02), statistically lower nitrogen (0.1% ± 0.03% for BPF1 vs. 0.07% ± 0.02% for BPF2, p < 10–6). The d13C values for inorganic carbon did not correlate with those of organic carbon in BPF2, suggesting lower heterotrophic respiration. An increase in the δ13C of inorganic carbon with depth either reflects an autotrophic signal or mixing between a heterotrophic source at the surface and a lithotrophic source at depth. Potential enzyme activity of xylosidase and N-acetyl-β-D-glucosaminidase increases twofold at 15°C, relative to 25°C, indicating cold adaptation in the cultures and bulk soil. Potential enzyme activity of leucine aminopeptidase across soils and cultures was two orders of magnitude higher than other tested enzymes, implying that organisms use leucine as a nitrogen and carbon source in this nutrient-limited environment. Besides demonstrating large variability in carbon compositions of permafrost active layer soils only ∼84 m apart, results suggest that the Svalbard active layer microbes are often limited by organic carbon or nitrogen availability and have adaptations to the current environment, and metabolic flexibility to adapt to the warming climate.eng
dc.language.isoengnone
dc.publisherHumboldt-Universität zu Berlin
dc.rights(CC BY 4.0) Attribution 4.0 Internationalger
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectSvalbardeng
dc.subjectpermafrosteng
dc.subjectactive layereng
dc.subjectcarboneng
dc.subjectnitrogeneng
dc.subjectmetagenomeeng
dc.subjectcultureseng
dc.subjectenzymeseng
dc.subject.ddc551 Geologie, Hydrologie, Meteorologienone
dc.titlePermafrost Active Layer Microbes From Ny Ålesund, Svalbard (79°N) Show Autotrophic and Heterotrophic Metabolisms With Diverse Carbon-Degrading Enzymesnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-110-18452/25086-9
dc.identifier.doi10.3389/fmicb.2021.757812none
dc.identifier.doihttp://dx.doi.org/10.18452/24432
dc.type.versionpublishedVersionnone
local.edoc.container-titleFrontiers in microbiologynone
local.edoc.pages14none
local.edoc.type-nameZeitschriftenartikel
local.edoc.institutionMathematisch-Naturwissenschaftliche Fakultätnone
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-publisher-nameFrontiers Medianone
local.edoc.container-publisher-placeLausannenone
local.edoc.container-volume12none
dc.description.versionPeer Reviewednone
local.edoc.container-articlenumber757812none
dc.identifier.eissn1664-302X

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