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2021-09-15Zeitschriftenartikel DOI: 10.18452/24579
Single-nucleus RNA sequencing of plant tissues using a nanowell‐based system
dc.contributor.authorSunaga-Franze, Daniele Yumi
dc.contributor.authorMuino, Jose M
dc.contributor.authorBraeuning, Caroline
dc.contributor.authorXu, Xiaocai
dc.contributor.authorZong, Minglei
dc.contributor.authorSmaczniak, Cezary
dc.contributor.authorYan, Wenhao
dc.contributor.authorFischer, Cornelius
dc.contributor.authorVidal, Ramon
dc.contributor.authorKliem, Magdalena
dc.contributor.authorKaufmann, Kerstin
dc.contributor.authorSauer, Sascha
dc.date.accessioned2022-04-29T10:47:42Z
dc.date.available2022-04-29T10:47:42Z
dc.date.issued2021-09-15none
dc.date.updated2022-03-21T03:36:57Z
dc.identifier.issn0960-7412
dc.identifier.urihttp://edoc.hu-berlin.de/18452/25253
dc.description.abstractSingle-cell genomics provides unprecedented potential for research on plant development and environmental responses. Here, we introduce a generic procedure for plant nucleus isolation combined with nanowell-based library preparation. Our method enables the transcriptome analysis of thousands of individual plant nuclei. It serves as an alternative to the use of protoplast isolation, which is currently a standard methodology for plant single-cell genomics, although it can be challenging for some plant tissues. We show the applicability of our nucleus isolation method by using different plant materials from different species. The potential of our single-nucleus RNA sequencing method is shown through the characterization of transcriptomes of seedlings and developing flowers from Arabidopsis thaliana. We evaluated the transcriptome dynamics during the early stages of anther development, identified stage-specific activities of transcription factors regulating this process, and predicted potential target genes of these transcription factors. Our nucleus isolation procedure can be applied in different plant species and tissues, thus expanding the toolkit for plant single-cell genomics experiments.eng
dc.language.isoengnone
dc.publisherHumboldt-Universität zu Berlin
dc.rights(CC BY-NC-ND 4.0) Attribution-NonCommercial-NoDerivatives 4.0 Internationalger
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectsingle‐nucleus isolationeng
dc.subjectsnRNA‐seqeng
dc.subjectArabidopsis thaliana seedlingseng
dc.subjectflower developmenteng
dc.subjectanthereng
dc.subjecttechnical advanceeng
dc.subject.ddc580 Pflanzen (Botanik)none
dc.subject.ddc570 Biologienone
dc.titleSingle-nucleus RNA sequencing of plant tissues using a nanowell‐based systemnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-110-18452/25253-5
dc.identifier.doihttp://dx.doi.org/10.18452/24579
dc.type.versionpublishedVersionnone
local.edoc.pages11none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
dc.description.versionPeer Reviewednone
dc.identifier.eissn1365-313X
dcterms.bibliographicCitation.doi10.1111/tpj.15458none
dcterms.bibliographicCitation.journaltitleThe plant journalnone
dcterms.bibliographicCitation.volume108none
dcterms.bibliographicCitation.issue3none
dcterms.bibliographicCitation.originalpublishernameWiley-Blackwellnone
dcterms.bibliographicCitation.originalpublisherplaceOxford [u.a.]none
dcterms.bibliographicCitation.pagestart859none
dcterms.bibliographicCitation.pageend869none
bua.departmentLebenswissenschaftliche Fakultätnone

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