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2020-06-12Zeitschriftenartikel DOI: 10.3389/fcell.2020.00337
Liquid-Ordered Phase Formation by Mammalian and Yeast Sterols: A Common Feature With Organizational Differences
dc.contributor.authorKhmelinskaia, Alena
dc.contributor.authorTrigo Marquês, Joaquim
dc.contributor.authorBastos, André E. P.
dc.contributor.authorAntunes, Catarina
dc.contributor.authorOliveira, Andreia
dc.contributor.authorScolari, Silvia
dc.contributor.authorLobo, Gerson M. da S.
dc.contributor.authorMalhó, Rui
dc.contributor.authorHerrmann, Andreas
dc.contributor.authorMarinho, H. Susana
dc.contributor.authorde Almeida, Rodrigo F. M.
dc.date.accessioned2022-05-11T12:03:19Z
dc.date.available2022-05-11T12:03:19Z
dc.date.issued2020-06-12none
dc.date.updated2020-06-12T23:07:48Z
dc.identifier.urihttp://edoc.hu-berlin.de/18452/25328
dc.description.abstractHere, biophysical properties of membranes enriched in three metabolically related sterols are analyzed both in vitro and in vivo. Unlike cholesterol and ergosterol, the common metabolic precursor zymosterol is unable to induce the formation of a liquid ordered (lo) phase in model lipid membranes and can easily accommodate in a gel phase. As a result, Zym has a marginal ability to modulate the passive membrane permeability of lipid vesicles with different compositions, contrary to cholesterol and ergosterol. Using fluorescence-lifetime imaging microscopy of an aminostyryl dye in living mammalian and yeast cells we established a close parallel between sterol-dependent membrane biophysical properties in vivo and in vitro. This approach unraveled fundamental differences in yeast and mammalian plasma membrane organization. It is often suggested that, in eukaryotes, areas that are sterol-enriched are also rich in sphingolipids, constituting highly ordered membrane regions. Our results support that while cholesterol is able to interact with saturated lipids, ergosterol seems to interact preferentially with monounsaturated phosphatidylcholines. Taken together, we show that different eukaryotic kingdoms developed unique solutions for the formation of a sterol-rich plasma membrane, a common evolutionary trait that accounts for sterol structural diversity.eng
dc.language.isoengnone
dc.publisherHumboldt-Universität zu Berlin
dc.rights(CC BY 4.0) Attribution 4.0 Internationalger
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectergosteroleng
dc.subjectcholesteroleng
dc.subjectzymosteroleng
dc.subjectfluorescence lifetime imaging microscopyeng
dc.subjectfluorescence spectroscopyeng
dc.subjectplasma membraneeng
dc.subjectlipid-lipid interactionseng
dc.subjectsterol-rich domaineng
dc.subject.ddc570 Biologienone
dc.titleLiquid-Ordered Phase Formation by Mammalian and Yeast Sterols: A Common Feature With Organizational Differencesnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-110-18452/25328-3
dc.identifier.doi10.3389/fcell.2020.00337none
dc.identifier.doihttp://dx.doi.org/10.18452/24663
dc.type.versionpublishedVersionnone
local.edoc.container-titleFrontiers in Cell and Developmental Biologynone
local.edoc.pages15none
local.edoc.type-nameZeitschriftenartikel
local.edoc.institutionLebenswissenschaftliche Fakultätnone
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-publisher-nameFrontiers Medianone
local.edoc.container-publisher-placeLausannenone
local.edoc.container-volume8none
dc.description.versionPeer Reviewednone
local.edoc.container-articlenumber337none
dc.identifier.eissn2296-634X

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