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2022-02-23Zeitschriftenartikel DOI: 10.18452/24747
Characterization of Hantavirus N Protein Intracellular Dynamics and Localization
dc.contributor.authorWelke, Robert-William
dc.contributor.authorSperber, Hannah Sabeth
dc.contributor.authorBergmann, Ronny
dc.contributor.authorKoikkarah, Amit
dc.contributor.authorMenke, Laura
dc.contributor.authorSieben, Christian
dc.contributor.authorKrüger, Detlev H.
dc.contributor.authorChiantia, Salvatore
dc.contributor.authorHerrmann, Andreas
dc.contributor.authorSchwarzer, Roland
dc.date.accessioned2022-06-09T11:52:14Z
dc.date.available2022-06-09T11:52:14Z
dc.date.issued2022-02-23none
dc.date.updated2022-03-23T08:29:19Z
dc.identifier.urihttp://edoc.hu-berlin.de/18452/25422
dc.description.abstractHantaviruses are enveloped viruses that possess a tri-segmented, negative-sense RNA genome. The viral S-segment encodes the multifunctional nucleocapsid protein (N), which is involved in genome packaging, intracellular protein transport, immunoregulation, and several other crucial processes during hantavirus infection. In this study, we generated fluorescently tagged N protein constructs derived from Puumalavirus (PUUV), the dominant hantavirus species in Central, Northern, and Eastern Europe. We comprehensively characterized this protein in the rodent cell line CHO-K1, monitoring the dynamics of N protein complex formation and investigating co-localization with host proteins as well as the viral glycoproteins Gc and Gn. We observed formation of large, fibrillar PUUV N protein aggregates, rapidly coalescing from early punctate and spike-like assemblies. Moreover, we found significant spatial correlation of N with vimentin, actin, and P-bodies but not with microtubules. N constructs also co-localized with Gn and Gc albeit not as strongly as the glycoproteins associated with each other. Finally, we assessed oligomerization of N constructs, observing efficient and concentration-dependent multimerization, with complexes comprising more than 10 individual proteins.eng
dc.language.isoengnone
dc.publisherHumboldt-Universität zu Berlin
dc.rights(CC BY 4.0) Attribution 4.0 Internationalger
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjecthantaviruseng
dc.subjectN proteineng
dc.subjectoligomerizationeng
dc.subjectactineng
dc.subjectP-bodieseng
dc.subjectvimentineng
dc.subjectNumber and Brightnesseng
dc.subjectPuumalaviruseng
dc.subjectmacromolecular assemblieseng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.subject.ddc570 Biologienone
dc.titleCharacterization of Hantavirus N Protein Intracellular Dynamics and Localizationnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-110-18452/25422-3
dc.identifier.doihttp://dx.doi.org/10.18452/24747
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
dc.identifier.eissn1999-4915
dcterms.bibliographicCitation.doi10.3390/v14030457none
dcterms.bibliographicCitation.journaltitleVirusesnone
dcterms.bibliographicCitation.volume14none
dcterms.bibliographicCitation.issue3none
dcterms.bibliographicCitation.articlenumber457none
dcterms.bibliographicCitation.originalpublishernameMDPInone
dcterms.bibliographicCitation.originalpublisherplaceBaselnone
bua.departmentLebenswissenschaftliche Fakultätnone

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