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2020-12-17Zeitschriftenartikel DOI: 10.3390/microorganisms8122024
The Role of Petrimonas mucosa ING2-E5AT in Mesophilic Biogas Reactor Systems as Deduced from Multiomics Analyses
dc.contributor.authorMaus, Irena
dc.contributor.authorTubbesing, Tom
dc.contributor.authorWibberg, Daniel
dc.contributor.authorHeyer, Robert
dc.contributor.authorHassa, Julia
dc.contributor.authorTomazetto, Geizecler
dc.contributor.authorHuang, Liren
dc.contributor.authorBunk, Boyke
dc.contributor.authorSpröer, Cathrin
dc.contributor.authorBenndorf, Dirk
dc.contributor.authorZverlov, Vladimir
dc.contributor.authorPühler, Alfred
dc.contributor.authorKlocke, Michael
dc.contributor.authorSczyrba, Alexander
dc.contributor.authorSchlüter, Andreas
dc.date.accessioned2022-08-09T12:55:35Z
dc.date.available2022-08-09T12:55:35Z
dc.date.issued2020-12-17none
dc.date.updated2021-01-08T02:36:05Z
dc.identifier.urihttp://edoc.hu-berlin.de/18452/25789
dc.description.abstractMembers of the genera Proteiniphilum and Petrimonas were speculated to represent indicators reflecting process instability within anaerobic digestion (AD) microbiomes. Therefore, Petrimonas mucosa ING2-E5AT was isolated from a biogas reactor sample and sequenced on the PacBio RSII and Illumina MiSeq sequencers. Phylogenetic classification positioned the strain ING2-E5AT in close proximity to Fermentimonas and Proteiniphilum species (family Dysgonomonadaceae). ING2-E5AT encodes a number of genes for glycosyl-hydrolyses (GH) which are organized in Polysaccharide Utilization Loci (PUL) comprising tandem susCD-like genes for a TonB-dependent outer-membrane transporter and a cell surface glycan-binding protein. Different GHs encoded in PUL are involved in pectin degradation, reflecting a pronounced specialization of the ING2-E5AT PUL systems regarding the decomposition of this polysaccharide. Genes encoding enzymes participating in amino acids fermentation were also identified. Fragment recruitments with the ING2-E5AT genome as a template and publicly available metagenomes of AD microbiomes revealed that Petrimonas species are present in 146 out of 257 datasets supporting their importance in AD microbiomes. Metatranscriptome analyses of AD microbiomes uncovered active sugar and amino acid fermentation pathways for Petrimonas species. Likewise, screening of metaproteome datasets demonstrated expression of the Petrimonas PUL-specific component SusC providing further evidence that PUL play a central role for the lifestyle of Petrimonas species.eng
dc.language.isoengnone
dc.publisherHumboldt-Universität zu Berlin
dc.rights(CC BY 4.0) Attribution 4.0 Internationalger
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectcarbohydrate-active enzymeseng
dc.subjectpolysaccharide utilization locieng
dc.subjectanaerobic digestioneng
dc.subjectbiomethanationeng
dc.subjectmetabolic pathway reconstructioneng
dc.subjectbioconversioneng
dc.subject.ddc570 Biologienone
dc.titleThe Role of Petrimonas mucosa ING2-E5AT in Mesophilic Biogas Reactor Systems as Deduced from Multiomics Analysesnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-110-18452/25789-8
dc.identifier.doi10.3390/microorganisms8122024none
dc.identifier.doihttp://dx.doi.org/10.18452/25110
dc.type.versionpublishedVersionnone
local.edoc.container-titleMicroorganismsnone
local.edoc.pages23none
local.edoc.type-nameZeitschriftenartikel
local.edoc.institutionHumboldt-Universität (insgesamt)none
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-publisher-nameMDPInone
local.edoc.container-publisher-placeBaselnone
local.edoc.container-volume8none
local.edoc.container-issue12none
dc.description.versionPeer Reviewednone
dc.identifier.eissn2076-2607

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