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2019-08-21Zeitschriftenartikel

Absorption and Emission Spectroscopic Investigation of the Thermal Dynamics of the Archaerhodopsin 3 Based Fluorescent Voltage Sensor QuasAr1

Penzkofer, Alfons; Silapetere, Arita; Hegemann, Peter

QuasAr1 is a fluorescent voltage sensor derived from Archaerhodopsin 3 (Arch) of Halorubrum sodomense by directed evolution. Here we report absorption and emission spectroscopic studies of QuasAr1 in Tris buffer at pH 8. ...

2017-10-05Zeitschriftenartikel

Absorption and Emission Spectroscopic Investigation of Thermal Dynamics and Photo-Dynamics of the Rhodopsin Domain of the Rhodopsin-Guanylyl Cyclase from the Nematophagous Fungus Catenaria anguillulae

Penzkofer, Alfons; Scheib, Ulrike; Stehfest, Katja; Hegemann, Peter

The rhodopsin-guanylyl cyclase from the nematophagous fungus Catenaria anguillulae belongs to a recently discovered class of enzymerhodopsins and may find application as a tool in optogenetics. Here the rhodopsin domain ...

2020-09-08Zeitschriftenartikel

Absorption and Emission Spectroscopic Investigation of the Thermal Dynamics of the Archaerhodopsin 3 Based Fluorescent Voltage Sensor Archon2

Penzkofer, Alfons; Silapetere, Arita; Hegemann, Peter

Archon2 is a fluorescent voltage sensor derived from Archaerhodopsin 3 (Arch) of Halorubrum sodomense using robotic multidimensional directed evolution approach. Here we report absorption and emission spectroscopic studies ...

2019-12-25Zeitschriftenartikel

Photocycle Dynamics of the Archaerhodopsin 3 Based Fluorescent Voltage Sensor QuasAr1

Penzkofer, Alfons; Silapetere, Arita; Hegemann, Peter

The retinal photocycle dynamics of the fluorescent voltage sensor QuasAr1 (Archaerhodopsin 3 P60S-T80S-D95H-D106H-F161V mutant from Halorubrum sodomense) in pH 8 Tris buffer was studied. The samples were photoexcited to ...

2021-06-23Zeitschriftenartikel

Comparative Anti-Inflammatory Effects of Salix Cortex Extracts and Acetylsalicylic Acid in SARS-CoV-2 Peptide and LPS-Activated Human In Vitro Systems

Le, Nguyen Phan Khoi; Herz, Corinna; Gomes, João Victor Dutra; Förster, Nadja; Antoniadou, Kyriaki; Mittermeier-Kleßinger, Verena Karolin; Mewis, Inga; Dawid, Corinna; Ulrichs, Christian; Lamy, Evelyn

The usefulness of anti-inflammatory drugs as an adjunct therapy to improve outcomes in COVID-19 patients is intensely discussed. Willow bark (Salix cortex) has been used for centuries to relieve pain, inflammation, and ...

2019-05-11Zeitschriftenartikel

Computational Aminoacyl-tRNA Synthetase Library Design for Photocaged Tyrosine

Baumann, Tobias; Hauf, Matthias; Richter, Florian; Albers, Suki; Möglich, Andreas; Ignatova, Zoya; Budisa, Nediljko

Engineering aminoacyl-tRNA synthetases (aaRSs) provides access to the ribosomal incorporation of noncanonical amino acids via genetic code expansion. Conventional targeted mutagenesis libraries with 5–7 positions randomized ...

2021-07-03Zeitschriftenartikel

Exogenous Applications of Bio-fabricated Silver Nanoparticles to Improve Biochemical, Antioxidant, Fatty Acid and Secondary Metabolite Contents of Sunflower

Batool, Syeda Umber; Javed, Bilal; Sohail; Zehra, Syeda Sadaf; Mashwani, Zia-ur-Rehman; Raja, Naveed Iqbal; Khan, Tariq; ALHaithloul, Haifa Abdulaziz Sakit; Alghanem, Suliman Mohammed; Al-Mushhin, Amina A. M.; Hashem, Mohamed; Alamri, Saad

The present study involved the bio-fabrication of silver nanoparticles (AgNPs) by using the Euphorbia helioscopia L. leaves aqueous extract to improve the production of secondary metabolites in industrially important ...

2021-03-29Zeitschriftenartikel

Structure, gating and interactions of the voltage-dependent anion channel

Najbauer, Eszter E.; Becker, Stefan; Giller, Karin; Zweckstetter, Markus; Lange, Adam; Steinem, Claudia; de Groot, Bert; Griesinger, Christian; Andreas, Loren B.

The voltage-dependent anion channel (VDAC) is one of the most highly abundant proteins found in the outer mitochondrial membrane, and was one of the earliest discovered. Here we review progress in understanding VDAC function ...

2020-10-14Zeitschriftenartikel

Systemweite Anreicherung von RNA-Protein-Komplexen

Pfister, Stefan; Beckmann, Benedikt

RNA-protein complexes (RNPs) are key players in cell physiology, especially in the context of RNA metabolism. However, their analysis has been dependent on specific protein epitopes or RNA sequence elements, preventing ...

2023-02-21Zeitschriftenartikel

Plant organellar RNA maturation

Small, Ian; Melonek, Joanna; Bohne, Alexandra Viola; Nickelsen, Joerg; Schmitz-Linneweber, Christian

Plant organellar RNA metabolism is run by a multitude of nucleus-encoded RNA-binding proteins (RBPs) that control RNA stability, processing, and degradation. In chloroplasts and mitochondria, these post-transcriptional ...