Show simple item record

2017-08-31Zeitschriftenartikel DOI: 10.18452/25316
Stable Membrane-Association of mRNAs in Etiolated, Greening and Mature Plastids
dc.contributor.authorLegen, Julia
dc.contributor.authorSchmitz-Linneweber, Christian
dc.date.accessioned2022-09-15T16:23:55Z
dc.date.available2022-09-15T16:23:55Z
dc.date.issued2017-08-31none
dc.date.updated2022-09-05T15:49:31Z
dc.identifier.urihttp://edoc.hu-berlin.de/18452/26009
dc.description.abstractChloroplast genes are transcribed as polycistronic precursor RNAs that give rise to a multitude of processing products down to monocistronic forms. Translation of these mRNAs is realized by bacterial type 70S ribosomes. A larger fraction of these ribosomes is attached to chloroplast membranes. This study analyzed transcriptome-wide distribution of plastid mRNAs between soluble and membrane fractions of purified plastids using microarray analyses and validating RNA gel blot hybridizations. To determine the impact of light on mRNA localization, we used etioplasts, greening plastids and mature chloroplasts from Zea mays as a source for membrane and soluble extracts. The results show that the three plastid types display an almost identical distribution of RNAs between the two organellar fractions, which is confirmed by quantitative RNA gel blot analyses. Furthermore, they reveal that different RNAs processed from polycistronic precursors show transcript-autonomous distribution between stroma and membrane fractions. Disruption of ribosomes leads to release of mRNAs from membranes, demonstrating that attachment is likely a direct consequence of translation. We conclude that plastid mRNA distribution is a stable feature of different plastid types, setting up rapid chloroplast translation in any plastid type.eng
dc.description.sponsorshipDeutsche Forschungsgemeinschaft
dc.language.isoengnone
dc.publisherHumboldt-Universität zu Berlin
dc.rights(CC BY 4.0) Attribution 4.0 Internationalger
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectchloroplasteng
dc.subjectetioplasteng
dc.subjectmembraneeng
dc.subjectorganelleeng
dc.subjectribosomeeng
dc.subjectRNA processingeng
dc.subjecttranslationeng
dc.subjectZea mayseng
dc.subject.ddc570 Biologienone
dc.titleStable Membrane-Association of mRNAs in Etiolated, Greening and Mature Plastidsnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-110-18452/26009-8
dc.identifier.doihttp://dx.doi.org/10.18452/25316
dc.type.versionpublishedVersionnone
local.edoc.pages15none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
dc.description.versionPeer Reviewednone
dc.identifier.eissn1422-0067
dcterms.bibliographicCitation.doi10.3390/ijms18091881none
dcterms.bibliographicCitation.journaltitleInternational journal of molecular sciencesnone
dcterms.bibliographicCitation.volume18none
dcterms.bibliographicCitation.issue9none
dcterms.bibliographicCitation.articlenumber1881none
dcterms.bibliographicCitation.originalpublishernameMolecular Diversity Preservation Internationalnone
dcterms.bibliographicCitation.originalpublisherplaceBaselnone
bua.departmentLebenswissenschaftliche Fakultätnone

Show simple item record