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2022-06-13Zeitschriftenartikel DOI: 10.1111/tpj.15865
Chlorophyll dephytylase 1 and chlorophyll synthase: a chlorophyll salvage pathway for the turnover of photosystems I and II
dc.contributor.authorLin, Yao‐Pin
dc.contributor.authorShen, Yu‐Yen
dc.contributor.authorShiu, Yen‐Bin
dc.contributor.authorCharng, Yee-yung
dc.contributor.authorGrimm, Bernhard
dc.date.accessioned2022-10-27T14:14:49Z
dc.date.available2022-10-27T14:14:49Z
dc.date.issued2022-06-13none
dc.date.updated2022-09-21T22:39:49Z
dc.identifier.urihttp://edoc.hu-berlin.de/18452/26109
dc.description.abstractChlorophyll (Chl) is made up of the tetrapyrrole chlorophyllide and phytol, a diterpenoid alcohol. The photosynthetic protein complexes utilize Chl for light harvesting to produce biochemical energy for plant development. However, excess light and adverse environmental conditions facilitate generation of reactive oxygen species, which damage photosystems I and II (PSI and PSII) and induce their turnover. During this process, Chl is released, and is thought to be recycled via dephytylation and rephytylation. We previously demonstrated that Chl recycling in Arabidopsis under heat stress is mediated by the enzymes chlorophyll dephytylase 1 (CLD1) and chlorophyll synthase (CHLG) using chlg and cld1 mutants. Here, we show that the mutants with high CLD1/CHLG ratio, by different combinations of chlg‐1 (a knock‐down mutant) and the hyperactive cld1‐1 alleles, develop necrotic leaves when grown under long‐ and short‐day, but not continuous light conditions, owing to the accumulation of chlorophyllide in the dark. Combination of chlg‐1 with cld1‐4 (a knock‐out mutant) leads to reduced chlorophyllide accumulation and necrosis. The operation of CLD1 and CHLG as a Chl salvage pathway was also explored in the context of Chl recycling during the turnover of Chl‐binding proteins of the two photosystems. CLD1 was found to interact with CHLG and the light‐harvesting complex‐like proteins OHP1 and LIL3, implying that auxiliary factors are required for this process.eng
dc.description.sponsorshipDeutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659
dc.description.sponsorshipMinistry of Science and Technology, Taiwan http://dx.doi.org/10.13039/501100004663
dc.language.isoengnone
dc.publisherHumboldt-Universität zu Berlin
dc.rights(CC BY 4.0) Attribution 4.0 Internationalger
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectchlorophyll salvageeng
dc.subjectchlorophyll dephytylase 1eng
dc.subjectchlorophyll synthaseeng
dc.subjectlight/dark cycleeng
dc.subjectphotosystem turnovereng
dc.subjecttetrapyrrole biosynthesiseng
dc.subjectphotosynthesiseng
dc.subject.ddc580 Pflanzen (Botanik)none
dc.titleChlorophyll dephytylase 1 and chlorophyll synthase: a chlorophyll salvage pathway for the turnover of photosystems I and IInone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-110-18452/26109-3
dc.identifier.doi10.1111/tpj.15865none
dc.identifier.doihttp://dx.doi.org/10.18452/25418
dc.type.versionpublishedVersionnone
local.edoc.container-titleThe plant journalnone
local.edoc.pages16none
local.edoc.type-nameZeitschriftenartikel
local.edoc.institutionLebenswissenschaftliche Fakultätnone
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-publisher-nameWiley-Blackwellnone
local.edoc.container-publisher-placeOxford [u.a.]none
local.edoc.container-volume111none
local.edoc.container-issue4none
local.edoc.container-firstpage979none
local.edoc.container-lastpage994none
dc.description.versionPeer Reviewednone
dc.identifier.eissn1365-313X

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