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2022-12-20Zeitschriftenartikel DOI: 10.3389/fvets.2022.1078394
miRNA profiling in intrauterine exosomes of pregnant cattle on day 7
dc.contributor.authorZhai, Yaying
dc.contributor.authorShi, Qiaoting
dc.contributor.authorChu, Qiuxia
dc.contributor.authorChen, Fuying
dc.contributor.authorFeng, Yajie
dc.contributor.authorZhang, Zijing
dc.contributor.authorQi, Xinglei
dc.contributor.authorArends, Danny
dc.contributor.authorBrockmann, Gudrun A
dc.contributor.authorWang, Eryao
dc.contributor.authorLyu, Shijie
dc.date.accessioned2023-01-24T13:43:17Z
dc.date.available2023-01-24T13:43:17Z
dc.date.issued2022-12-20none
dc.date.updated2023-01-17T06:26:47Z
dc.identifier.urihttp://edoc.hu-berlin.de/18452/26631
dc.description.abstractIntrauterine exosomes have been identified to be involved in the embryo development and implantation. The aim of this study was to explore the role of miRNAs in intrauterine exosomes in bovine pregnancy. Intrauterine exosomes were collected from uterine flushing fluids of three donor and three recipient Xianan cows 7 days after fertilization. Intrauterine exosomes miRNAs were extracted and the exosomal miRNAs expression levels were analyzed. Sixty miRNAs differed significantly in their amounts between donors and recipients (p-value < 0.05, |log2(FoldChange)| > 1). Twenty-two miRNAs were upregulated and 38 downregulated in the group of donor cows. The bta-miR-184 was the most significant (PBenjamini-Hochberg < 0.001). A total of 9,775 target genes were predicted using the 60 miRNAs. GO and KEGG analysis showed that the target genes were enriched in several biological processes or pathways associated with embryo implantation and endometrial development, such as cell adhesion, cell junction, focal adhesion, and Rap1 signaling pathway. Our findings suggest that, in cattle early pregnancy stage, these differently expressed miRNAs in intrauterine exosomes involved in embryo implantation and endometrial development, which may exert a significant effect and influence the uterine microenvironment for embryo implantation. These results could provide reference for screening and exploring the intrauterine exosomal miRNA affecting embryo implantation.eng
dc.language.isoengnone
dc.publisherHumboldt-Universität zu Berlin
dc.rights(CC BY 4.0) Attribution 4.0 Internationalger
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectbovineeng
dc.subjectuterine flushingeng
dc.subjectreceptive endometriumeng
dc.subjectapoptosiseng
dc.subjectproliferationeng
dc.subject.ddc630 Landwirtschaft und verwandte Bereichenone
dc.titlemiRNA profiling in intrauterine exosomes of pregnant cattle on day 7none
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-110-18452/26631-0
dc.identifier.doi10.3389/fvets.2022.1078394none
dc.identifier.doihttp://dx.doi.org/10.18452/25934
dc.type.versionpublishedVersionnone
local.edoc.container-titleFrontiers in veterinary sciencenone
local.edoc.pages9none
local.edoc.type-nameZeitschriftenartikel
local.edoc.institutionLebenswissenschaftliche Fakultätnone
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-publisher-nameFrontiers Research Foundationnone
local.edoc.container-publisher-placeLausannenone
local.edoc.container-volume9none
dc.description.versionPeer Reviewednone
local.edoc.container-articlenumber1078394none
dc.identifier.eissn2297-1769

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