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2023-12-20Zeitschriftenartikel DOI: 10.18452/28567
Arabidopsis thaliana GLYCINE RICH RNA‐BINDING PROTEIN 7 interaction with its iCLIP target LHCB1.1 correlates with changes in RNA stability and circadian oscillation
dc.contributor.authorLewinski, Martin
dc.contributor.authorSteffen, Alexander
dc.contributor.authorKachariya, Nitin
dc.contributor.authorElgner, Mareike
dc.contributor.authorSchmal, Christoph
dc.contributor.authorMessini, Niki
dc.contributor.authorKöster, Tino
dc.contributor.authorReichel, Marlene
dc.contributor.authorSattler, Michael
dc.contributor.authorZarnack, Kathi
dc.contributor.authorStaiger, Dorothee
dc.date.accessioned2024-04-23T16:26:25Z
dc.date.available2024-04-23T16:26:25Z
dc.date.issued2023-12-20none
dc.date.updated2024-04-16T08:21:56Z
dc.identifier.issn0960-7412
dc.identifier.urihttp://edoc.hu-berlin.de/18452/29201
dc.description.abstractThe importance of RNA‐binding proteins (RBPs) for plant responses to environmental stimuli and development is well documented. Insights into the portfolio of RNAs they recognize, however, clearly lack behind the understanding gathered in non‐plant model organisms. Here, we characterize binding of the circadian clock‐regulated Arabidopsis thaliana GLYCINE‐RICH RNA‐BINDING PROTEIN 7 (AtGRP7) to its target transcripts. We identified novel RNA targets from individual‐nucleotide resolution UV crosslinking and immunoprecipitation (iCLIP) data using an improved bioinformatics pipeline that will be broadly applicable to plant RBP iCLIP data. 2705 transcripts with binding sites were identified in plants expressing AtGRP7‐GFP that were not recovered in plants expressing an RNA‐binding dead variant or GFP alone. A conserved RNA motif enriched in uridine residues was identified at the AtGRP7 binding sites. NMR titrations confirmed the preference of AtGRP7 for RNAs with a central U‐rich motif. Among the bound RNAs, circadian clock‐regulated transcripts were overrepresented. Peak abundance of the LHCB1.1 transcript encoding a chlorophyll‐binding protein was reduced in plants overexpressing AtGRP7 whereas it was elevated in atgrp7 mutants, indicating that LHCB1.1 was regulated by AtGRP7 in a dose‐dependent manner. In plants overexpressing AtGRP7, the LHCB1.1 half‐life was shorter compared to wild‐type plants whereas in atgrp7 mutant plants, the half‐life was significantly longer. Thus, AtGRP7 modulates circadian oscillations of its in vivo binding target LHCB1.1 by affecting RNA stability.eng
dc.description.sponsorshipDeutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659
dc.language.isoengnone
dc.publisherHumboldt-Universität zu Berlin
dc.rights(CC BY-NC 4.0) Attribution-NonCommercial 4.0 Internationalger
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.subjectArabidopsiseng
dc.subjectiCLIPeng
dc.subjectRNA‐binding proteineng
dc.subjectRNA‐protein interactioneng
dc.subjectPureCLIP scoreeng
dc.subjectcircadianeng
dc.subject.ddc570 Biologienone
dc.titleArabidopsis thaliana GLYCINE RICH RNA‐BINDING PROTEIN 7 interaction with its iCLIP target LHCB1.1 correlates with changes in RNA stability and circadian oscillationnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-110-18452/29201-1
dc.identifier.doihttp://dx.doi.org/10.18452/28567
dc.type.versionpublishedVersionnone
local.edoc.pages22none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
dc.description.versionPeer Reviewednone
dc.identifier.eissn1365-313X
dcterms.bibliographicCitation.doi10.1111/tpj.16601
dcterms.bibliographicCitation.journaltitleThe plant journalnone
dcterms.bibliographicCitation.volume118none
dcterms.bibliographicCitation.issue1none
dcterms.bibliographicCitation.originalpublishernameWiley-Blackwellnone
dcterms.bibliographicCitation.originalpublisherplaceOxford [u.a.]none
dcterms.bibliographicCitation.pagestart203none
dcterms.bibliographicCitation.pageend224none
bua.departmentLebenswissenschaftliche Fakultätnone

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