TuPPL: Tub-tag mediated C-terminal protein–protein-ligation using complementary click-chemistry handles
Mathematisch-Naturwissenschaftliche Fakultät
We introduce a chemoenzymatic strategy for straightforward in vitro generation of C-terminally linked fusion proteins. Tubulin tyrosine ligase is used for the incorporation of complementary click chemistry handles facilitating subsequent formation of functional bispecific antibody-fragments. This simple strategy may serve as central conjugation hub for a modular protein ligation platform.
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