El-Zend, Manar A.El-Deen, Ibrahim M.Mansour, Rawda M.Yousef, Tarek A.Alrashidi, Amal AbdullahSaied, Essa M.2025-12-102025-12-102025-08-222025-11-28http://edoc.hu-berlin.de/18452/36185Cancer remains a significant global health concern, with breast cancer ranking among the leading causes of cancer-related mortality in women. In pursuit of multi-targeted anticancer agents, we designed and synthesized a novel series of 7-hydroxy azacoumarin–α-cyanocinnamate hybrids and evaluated their therapeutic potential through comprehensive in vitro and in vivo studies. Structural characterization was confirmed using NMR, IR, and elemental analysis. Among the synthesized compounds, compound 7 exhibited the most potent cytotoxic activity against MCF-7 cells (IC50 = 7.65 μM) and MDA-MB-231 (IC50 = 9.7 ± 1.15 μM), with notable selectivity over non-tumorigenic MCF-10A cells (IC50 = 52.02 μM), as compared to the reference drug doxorubicin. Mechanistic in vitro investigations revealed that compound 7 induced G2/M phase arrest and apoptosis, accompanied by upregulation of pro-apoptotic markers (Bax, p53) and suppression of Bcl-2. Additionally, compound 7 significantly inhibited tubulin polymerization and demonstrated marked antioxidant activity in the FRAP assay (IC50 = 144.71 μM), as well as selective COX-2 inhibition (IC50 = 1.264 μM, SI = 5.93). In vivo evaluation using the Ehrlich ascites carcinoma (EAC) model confirmed its anticancer efficacy, with 85.92% reduction in viable EAC cells and substantial tumor volume suppression at 10 mg kg−1. Notably, compound 7 mitigated EAC-induced hepatorenal toxicity by restoring liver and kidney biomarkers and reducing oxidative stress and lipid peroxidation. Furthermore, it significantly downregulated pro-inflammatory (TNF-α) and angiogenic (VEGFR-II) markers while preserving normal tissue histoarchitecture. Collectively, these findings highlight compound 7 as a promising multi-functional lead candidate with cytotoxic, antioxidant, anti-inflammatory, and anti-angiogenic activities, meriting further development in cancer therapeutics.eng(CC BY 3.0) Attribution 3.0 Unported540 Chemie und zugeordnete Wissenschaftenarticleurn:nbn:de:kobv:11-110-18452/36185-910.18452/355342632-8682